VEGA 2/0044/13

Principle of gating of voltage-dependent calcium channels        


Principal Investigator: Ľubica Lacinová

Duration: January 2013 – December 2015
Coordinating Organization: Institute of Molecular Physiology and Genetics, Bratislava, Slovak Republic


Change of transmembrane voltage initiates activation, deactivation and inactivation of voltage-dependent ion channels including voltage-dependent calcium channels. This so-called gating precisely regulates entry of calcium ions into the cell. In addition to electrical excitability intracellular calcium ions control number of processes including secretion, contraction and gene transcription. How precisely gating machinery of an voltage-dependent channel turns change of transmembrane voltage into conformational changes of channel’s voltage sensor and conducting pore is not know. We will address function of voltage sensors in individual domains of CaV3 voltage-dependent calcium channel and the interaction of these sensors with channel’s conducting pore with the aim to identify determinants of activation threshold voltage.


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Pavlovičová M, Karmažínová M, Huláková S and Lacinová Ľ (2014): Bisphenol A differently inhibits CaV3.1, CaV3.2 and CaV3.3 calcium channels. Naunyn-Schmiedeberg’s Arch Pharmacol 387:153–163.
image Karmažínová M, Jašková K, Griač P, Perez-Reyes E, Lacinová Ľ (2015): Contrasting the roles of the I-II loop gating brake in CaV3.1 and CaV3.3 calcium channels. Pflugers Arch 467:2519-2527.
Tomasova L, Pavlovicova M, Malekova L, Misak A, Kristek F, Grman M, Cacanyiova S, Tomasek M, Tomaskova Z, Perry A, Wood ME, Lacinova L, Ondrias K, Whiteman M (2015): Effects of AP39, a novel triphenylphosphonium derivatised anethole dithiolethione hydrogen sulfide donor, on rat haemodynamic parameters and chloride and calcium Cav3 and RyR2 channels. Nitric Oxide 46:131-144.
Weiss N, Lacinova L (2016): T-type channels: release a brake, engage a gear. Channels 10:78-80.
image Ondacova K, Karmazinova M, Lazniewska J, Weiss N, Lacinova L (2016): Modulation of Cav3.2 T-type calcium channel permeability by asparagine-linked glycosylation. Channels 10:175-184.